Docking, Not Fusion, as the Rate-Limiting Step in a SNARE-Driven Vesicle Fusion Assay
نویسندگان
چکیده
منابع مشابه
Docking, not fusion, as the rate-limiting step in a SNARE-driven vesicle fusion assay.
In vitro vesicle fusion assays that monitor lipid mixing between t-SNARE and v-SNARE vesicles in bulk solution exhibit remarkably slow fusion on the nonphysiological timescale of tens of minutes to several hours. Here, single-vesicle, fluorescence resonance energy transfer-based assays cleanly separate docking and fusion steps for individual vesicle pairs containing full-length SNAREs. Docking ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.03.015